Figure 5.

Voltage-dependent gating of TRPV1 and chimeras expressed in HEK293 cells. Representative family of whole-cell currents elicited with a voltage protocol consisting of 50 ms depolarizing pulses from −120 up to 300 mV in steps of 20 mV. I–V relationships evoked at increasing concentrations of capsaicin are shown underneath the ionic currents. Ramps consisted of a voltage step of 300 ms from the holding potential of 0 to −120 mV, followed by 350 ms linear ramp up to 160 mV. Representative current traces from n ≥ 6 cells are shown. Whole-cell currents were obtained in symmetrical 150 mm NaCl, from a holding potential of 0 mV. Ctrl, I–V curves obtained in the absence of capsaicin.