Figure 1.

RNA silencing of PINK1 causes alteration in mitochondrial morphology in human cells. A, siRNA-mediated downregulation of PINK1. Untransfected HeLa cells (−), or cells transfected with a control siRNA (control), or an siRNA against PINK1 were analyzed for endogenous PINK1 protein levels. Cells were transfected at two different cell densities with 5, 15, or 100 pmol of siRNA (1×, 3×, or 20×) (pPINK1, precursor form; mPINK1, mature form). A cross-reactive protein is marked by an asterisk. B, Representative examples of normal or altered (truncated or fragmented) mitochondrial morphologies including higher magnifications are shown.