Figure 7.

Suppression of PUMA expression protects wild-type cortical neurons from camptothecin-induced cell death. Wild-type cortical neurons were transfected at day 1 after plating with either plasmid pLenti–U1–control or pLenti–U1–PUMA, which enables coexpression of the respective shRNA and GFP (see supplemental Material and Methods, available at www.jneurosci.org as supplemental material). At 48 h after transfection, neurons were treated with 2.5 μm CPT or DMSO (vehicle control) and processed for GFP fluorescence and nuclear staining (Hoechst 33258; blue) after 24 h. These results are representative of two independent experiments.