Figure 6.

Overexpression of ICA69 disrupts PICK1–GluR2 coclusters in 293T cells. HEK 293T cells were transfected with myc-ICA69, HA-PICK1, and GluR2 in different combination as indicated. Scale bar, 10 μm. A, When PICK1 (green) and GluR2 (red) were coexpressed in 293T cells, they formed many coclusters in the cytosol. B, When ICA69 (blue) was coexpressed with PICK1 and GluR2, these clusters completely disappeared. C, When ICA69 was transfected with GluR2 without PICK1, both ICA69 and GluR2 were diffuse in the cytosol. D, When ICA69, PICK1, and GluR2 were expressed alone, they were all diffusely localized in the cytosol.