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Side-chain property at position 538. The peak current responses to 1 μm capsaicin, pH 5.5, 0.1 μm capsaicin, and 0.1 μm capsaicin plus pH 6.5 from various mutations introduced at V538 were plotted. The leucine and isoleucine substitutions resulted in different low pH responses albeit similar capsaicin currents. Data were recorded from transiently transfected HEK293 cells at −60 mV. Error bars indicate SEM.
