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. 2007 Nov 14;27(46):12546–12554. doi: 10.1523/JNEUROSCI.3463-07.2007

Figure 3.

Figure 3.

The effect of Sema3A on the density and the area of synapsin I and PSD-95 clusters and on the morphology of spines in crmp1+/− and crmp1−/− cultured cortical neurons. A, The cultured crmp1+/− (a, b, e, f) or crmp1−/− (c, d, g, h) E18.5 cortical neurons at 11 d in vitro were applied with (b, d, f, h) or without (a, c, e, g) Sema3A (3 nm) for 24 h, fixed, and then double stained with Alexa594-labeled phalloidin (magenta) and anti-synapsin I (green; a–d) or anti-PSD-95 (green; e–h) antibodies. Arrowheads represent typical immunoreactive clusters at actin-rich protrusions. B, Quantitative analysis of the effects of Sema3A on the density of synapsin I (a) or PSD-95 (c) clusters, and the mean area of synapsin I (b) or PSD-95 (d) clusters in crmp1+/− and crmp1−/− cultured cortical neurons. C, Quantitative analysis of the effects of Sema3A on the density (a), the length (b), and the width (c) of phalloidin-positive spines in crmp1+/− and crmp1−/− cultured cortical neurons. The density was estimated as shown in Materials and Methods. Each value represents mean ± SEM from 60 neurons of three independent cultures. *p < 0.01 compared with vehicle control. Scale bars: 5 μm.