Figure 1.
Parvalbumin fused to nuclear export signal localizes to both cytoplasm and nucleus of LNV pacemaker neurons. Anti-Myc and anti-PDF double immunofluorescence of adult fly brains expressing Myc-tagged PV and nonexpressing controls. PDF neuropeptide is present in the cytoplasm of the cell bodies and processes of LNVs but not in the nucleus. Colocalization of anti-PDF and anti-Myc staining demonstrates cytoplasmic localization of PV. Anti-Myc staining is also present in the LNV nuclei.