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. 2007 Nov 14;27(46):12516–12530. doi: 10.1523/JNEUROSCI.2739-07.2007

Figure 1.

Figure 1.

SynCAM proteins are prominently expressed in brain. a, Specificity of antibodies raised for this study. HEK 293 cells were transfected with expression constructs encoding full-length mouse SynCAM 1–4. Total cell lysates were prepared, and equal lysate fractions were analyzed by immunoblotting (lanes 1–4). Twenty micrograms of adult rat forebrain proteins were analyzed as positive control (lane 5). Top to bottom, Immunoblots probed with antibodies raised against the purified, natively N-deglycosylated full-length SynCAM 1 extracellular domain (YUC8, raised in chicken), against an extracellular SynCAM 2 peptide (YU524, raised in rabbit), against an extracellular SynCAM 3 peptide (YU525, raised in rabbit), and against an intracellular SynCAM 4 peptide (YU591, raised in rabbit). The numbers on the left indicate positions of molecular weight markers, and arrowheads show the running positions of the indicated SynCAM proteins. b, Tissue profile of SynCAM expression. The indicated tissues were prepared from adult rats, and equal protein amounts were analyzed by immunoblotting for the proteins shown. Synaptotagmin 1 (Syt 1) served as brain-specific control, and the widely expressed VCP and actin were additional loading controls.