Figure 3.

SynCAM proteins are distinctly glycosylated and prominently expressed in brain during early postnatal development. a, SynCAM proteins are differentially glycosylated. A membrane fraction was prepared from adult rat forebrain, and equal protein amounts were subjected to enzymatic deglycosylation at 37°C with sialidase to remove sialic acids or PNGase F to remove N-linked carbohydrates. Control samples were incubated in parallel as indicated. In each lane, 30 μg of total brain protein was loaded. Immunoblots were probed with antibodies against SynCAM 1–4 or the N-glycosylated control protein N-cadherin as indicated. The numbers on the left indicate positions of molecular weight markers. b, Developmental expression profile of SynCAM proteins in brain. Total rat brain proteins were prepared at the indicated embryonic (E) and postnatal (P) days. Thirty micrograms of protein were analyzed per lane by immunoblotting for expression of SynCAM 1–4. VCP served as loading control. The numbers on the left indicate positions of molecular weight markers. Arrowheads show the running positions of the indicated SynCAM proteins in their predominantly adult (black) and postnatal (white) glycosylation forms.