Figure 6.

A, Representative photomicrographs of GnRH and Fos double-label IHC in the rostral hypothalamus. Black arrows indicate Fos-stained nuclei. Red arrows indicate GnRH-stained cytoplasm. Red arrows with black outlines indicate cells stained for both GnRH and Fos. B, Percentage of GnRH neurons coexpressing Fos protein in the A.M. and P.M. Different letters indicate significant difference (p < 0.01; A.M. WT, n = 7; A.M. KO, n = 7; P.M. WT plus vehicle or acyline, n = 14; P.M. KO vehicle or acyline, n = 18). Scale bars, 50 μm. Error bars indicate SEM.