Figure 1.

Age-related increase in Aβ immunoreactivity. Representative images show Aβ-IR in hippocampus CA1 (A–E), subiculum (Sub.; F–J), and layers IV and V of frontal cortex (F.Ctx.; K–O) from female 3xTg-AD mice at ages 3 months (B, G, L), 6 months (C, H, M), 9 months (D, I, N), and 12 months (E, J, O) and from WT female mice at age 6 months (A, F, K). Specific Aβ-IR is not observed in WT mice but in 3xTg-AD is apparent intraneuronally by age 3 months and extracellularly (arrows) beginning at age 9 months. Scale bar, 100 μm.