Figure 3.

CTF levels do not change across conditions in 3xTg-AD mice. Representative images show CTF-IR in the subiculum of 6-month-old WT female mice (A) and female 3xTg-AD mice at 3 months (B), 6 months (C), 9 months (D), and 12 months (E) of age. F, Quantification of CTF load across conditions shows high CTF levels in all 3xTg-AD groups that does not significantly vary by age (F = 0.14, p = 0.94). A similar, extranuclear distribution of CTF-IR is observed at high magnification (400×) in WT mice (G) and 3xTg-AD mice (H) that qualitatively differs from the more uniform and often punctate appearance of Aβ-IR in 3xTg-AD mice (I). Scale bar, 50 μm.