Figure 4.
SNAP and H2O2 applied to cytoplasmic surface increase ASIC2a single-channel activity in inside-out patches from CHO cells. Applied potential −40 mV. Zero current (closed state) shown by horizontal line. A, Single-channel activity during application of pH 6.0 to external surface of patches from transfected cell (2a, bottom traces) and control cell (WT, top trace). B, Similar traces in presence of SNAP (100 μm). C, Effect of H2O2 (0.05%).