Figure 3.

Administration of BDNF into the third ventricle (3V) rapidly induces neuronal activity in several hypothalamic nuclei. A–D, Representative coronal sections showing c-fos signal in the hypothalami of wild-type mice treated with saline (A, C) or BDNF (B, D) 45 min after treatment. Arrows denote c-fos-containing cells in the PVN in D. E, Quantification of total number of c-fos⁺ neurons in the PVN, VMH, DMH, LH, and Arc of wild-type mice treated with saline (open bars) or BDNF (closed bars) (n = 4; *p < 0.01). Counts represent total number of c-fos⁺ cells in three independent coronal levels of each of the studied regions determined from unilateral counts.