Figure 3.

DA agonists have no effect on GluR1 surface expression on DA neurons in VTA cultures. VTA cultures were incubated with media (control group), the D₂ receptor agonist quinpirole (Quin; 1 μm, 10 μm), DA (1 μm, 10 μm), or the D₁ receptor agonist SKF 81297 (SKF; 1 μm, 10 μm) for 10 min. Then surface GluR1 was labeled by incubating live cells with antibody recognizing the extracellular N-terminal domain of GluR1, followed by Cy3 conjugated secondary antibody. DA agonist-treated groups were not significantly different from the control group (n = 18–34; ANOVA, p > 0.05). Results are presented as the mean area of GluR1 staining, normalized to the control group.