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Regenerative axon growth is impaired in Avil-hPLAP homozygous trigeminal neurons. Trigeminal ganglia from P1 neonatal embryos were dissociated and cultured for 24 h. A, B, Representatives of wild-type (A) and homozygous (B) neurons stained with anti-neurofilament antibody are shown. C, D, Total axon length per cell (C) and the length of the longest axon in each cell (D) were calculated by MetaMorph software. The error bars represent ± SEM. *p < 0.03, **p < 0.001. Scale bar, 100 μm.
