Figure 8.
The partial replenishment of the SVZ stem cell niche in knock-out (ko) mice after AraC treatment is mainly performed by few spared Smoc/c and Smoc/n B cells. a, Quantification of the general level of proliferation in the SVZ in controls (tamoxifen-treated Smoc/c) and ko (tamoxifen-treated NestinCreER/+; Smoc/c) animals before the implantation of the pump (t = −7), in saline-treated controls and ko, and in AraC-treated controls and ko, soon after the removal of the pump (t = 0) and 1 week after pump removal (t = 7). b, Quantification of the ability to generate neurospheres in controls (tamoxifen-treated Smoc/c) and ko (tamoxifen-treated NestinCreER/+; Smoc/c) animals before the implantation of the pump (t = −7) in saline-treated controls and ko, and in AraC-treated controls and ko, soon after the removal of the pump (t = 0) and 1 week after pump removal (t = 7). c, Quantification of the numbers of β-Gal + cells, representing the expression pattern of Gli1, in controls (tamoxifen-treated Smoc/c;Gli1LacZ/+) and ko (tamoxifen-treated NestinCreER/+;Smoc/c;Gli1LacZ/+) animals before the implantation of the pump (t = −7), in AraC-treated controls and AraC-treated ko animals, soon after the removal of the pump (t = 0), one week after pump removal (t = 7) and 1 month after pump removal (t = 30). d, Results of PCR genotyping performed on individual neurospheres obtained from tamoxifen-treated NestinCreER/+; Smoc/c mice that received AraC treatment, at t = 0 and t = 7. Note that n/n means that both events of recombination occurred; c/n means that only one event of recombination occurred; c/c indicate that no recombination occurred within the Smo conditional alleles. In all panels t = −7 means without pump, t = 0 means soon after pump removal and t = 7 means 1 week after pump removal. Error bars represent SD. Statistics were performed by t test, p < 0.05. In a–c, n = 3 animals each; in d, t = 0, n(NSPHs) = 480, 3 animals; t = 7, n(NSPHs) = 405, 3 animals.