Figure 3.

Immune histology for sema3A and neurofilament in the human proximal nerve stump and neuroma. Sema3A (N15 antibody, green), neurofilament (2H3 antibody; red) and cell nuclei (Topro; blue) are visualized. a, Proximal nerve stump: sema3A expression was usually low and limited to the epineurium surrounding a fascicle. b, Proximal nerve stump: rarely, a weak sema3A signal was observed within a fascicle. c, Neuroma: sema3A is present in a diffuse pattern in the epineurial space in the neuroma, with the highest intensity of staining in the proximity of a minifascicle (arrowhead). d, Neuroma: sema3A surrounds a single axon. e, f, Neuroma: sema3A surrounds a minifascicle. A three-dimensional projection of a z-stack of Figure 2e is available at www.jneurosci.org as supplemental material. The arrows point at areas of sema3A expression, and the arrowhead points at a minifascicle. Scale bars: 20 μm.