Figure 2.

Single-cell RT-PCR indicates that YFP-16 neurons are glutamatergic or glycinergic, whereas GIN neurons are GABAergic. RT-PCR was performed on YFP-16 and GIN neurons from slice recordings. Neurons were assayed for five indicators of transmitter content: VGluT1, VGluT2, GlyT2, GAD65, and GAD67. Two neurons are shown from each line. A, YFP-16 neuron A is VGluT2+, whereas neuron B is GlyT2+. B, GIN neuron A expressed the GABAergic markers GAD67 and GAD65, as well as the glycinergic marker GlyT2; GIN neuron B expressed only GAD67 and GAD65. Right, RNA controls were 0.5 ng RNA extracts from whole mouse brain processed alongside single cells.