Table 3.

Glutamatergic and glycinergic YFP-16 neurons exhibit physiology similar to each other but not to glycinergic GIN neurons

	YFP-16		GIN
	VGluT2+	GlyT2+	GlyT2−	GlyT2+
AP width (ms)	0.52 ± 0.09	0.53 ± 0.08	0.78 ± 0.13	0.73 ± 0.13
AP half-width (ms)	0.20 ± 0.04	0.21 ± 0.04	0.33 ± 0.08	0.32 ± 0.07
AHP (mV)	19.9 ± 2.8	20.2 ± 2.0	27.0 ± 3.0*	23.8 ± 4.4*
Maximum derivative (V/s)	536 ± 117	494 ± 132	341 ± 70	340 ± 93
Minimum derivative (V/s)	472 ± 104	445 ± 136	255 ± 70	259 ± 94
ADP (V/s)	7.54 ± 5.58	5.3 ± 3.32	−2.09 ± 1.86	−1.48 ± 2.51
Threshold (mV)	−50.9 ± 2.6	−50.1 ± 2.2	n/a	n/a
Input resistance (MΩ)	46 ± 19*	67 ± 19*	248 ± 136	230 ± 127
Maximum firing rate (Hz)	398 ± 136	355 ± 82	145 ± 32	169 ± 68
Adaptation ratio @40 Hz	0.72 ± 0.12	0.79 ± 0.08	0.91 ± 0.08	0.92 ± 0.09
Gain <80 Hz (Hz/nA)	178 ± 63	222 ± 74	240 ± 71	239 ± 120
Gain >80 Hz (Hz/nA)	98 ± 37	134 ± 37	202 ± 64	185 ± 99
Gain/input resistance	2.27 ± 0.74	2.06 ± 0.44	0.97 ± 0.39	1.12 ± 0.44
Rebound firing (Hz)	52 ± 32*	21 ± 16*	11 ± 8	8 ± 7

Left columns indicate the physiology of YFP-16 neurons subjected to RT-PCR. Spike parameters, n = 9 (each group); cell parameters, n = 7–9. Right columns indicate the physiology of GIN neurons that underwent RT-PCR. Most neurons expressed both GAD67 and GAD65; the data here are divided into those neurons that did or did not coexpress GlyT2 (spike parameters, n = 14, each group; cell parameters, n = 11–14). Data represent a separate pool of neurons than those analyzed in Table 2. Asterisks indicate p < 0.05 compared to neurons from the same transgenic line. Values indicate mean ± SD.