Figure 4.

Interactions between domains of SK2 and CK2. a, GST pull-down experiments used the indicated fragments of the N-terminal domain of SK2 as baits to detect interactions with N-terminally His-tagged CK2 α or CK2 β. Specifically bound prey proteins were detected by Western blotting with an anti-His antibody. b, Same as in a but with GST-fusion proteins of the C-terminal domain of SK2 as baits and additional testing for interactions with the membrane proximal portion of the SK2 N-terminal domain. Subunit schematics represent the interactions detected by the pull-down results.