Table 2.

Mitochondrial respiration

	State 4o	State 3	RCR
Control	5.23 ± 0.63	31.86 ± 3.22	6.34 ± 0.48
Estradiol	6.02 ± 0.64	48.22 ± 3.69*	8.60 ± 0.79*

Respiratory rates of whole-brain mitochondria isolated from rats with or without in vivo 17β-estradiol (E₂, 30 μg/kg) or sesame oil vehicle control treatment in the presence of l-malate (5 mm), l-glutamate (5 mm), ADP (410 μm) to initiate state 3 respiration, and atractyloside to induce state 4_o respiration. The RCR was calculated as the ratio of state 3/state 4_o. The data represents mean ± SEM of six separate experiments (*p < 0.05 as compared with control; n = 6).