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. 2007 Dec 19;27(51):13938–13948. doi: 10.1523/JNEUROSCI.3055-07.2007

Figure 4.

Figure 4.

Position-dependent preferential outgrowth of E15 spinal cord neurites on membranes from P0 limb muscles treated with PI-PLC and enzyme buffer. Spinal cord slices cut from the cervical [rostral spinal cord (RSC)] or lumbar [caudal spinal cord (CSC)] enlargement were placed on alternating stripes of membrane derived from rostral muscles, or from caudal muscles which were pretreated with PI-PLC enzyme or buffer for controls. A, Columns show the percentage of cultures showing preferential outgrowth on rostral or caudal membranes treated with enzyme buffer. Numbers above each column indicate the number of cocultures observed for each category. A χ2 test for differences between rostral and caudal neurite outgrowth on buffer treated membranes is highly significant: p < 0.001. B, Columns show percentage of cultures showing preferential outgrowth on rostral or caudal membranes treated with PI-PLC enzyme. Numbers above each column indicate number of cocultures observed for each category. A χ2 test for overall differences between rostral and caudal neurite outgrowth on PI-PLC enzyme treated membranes is highly significant: p < 0.001 whereas a χ2 test for differences between rostral and caudal neurite outgrowth growing selectively on either PI-PLC enzyme treated membranes is not significant: p = 1. C, Columns show the percentage of cultures showing preferential outgrowth on rostral membranes treated with PI-PLC enzyme or buffer. Numbers above each column indicate number of cocultures observed for each category. A χ2 test for differences between rostral and caudal neurite outgrowth on PI-PLC enzyme or buffer treated rostral membranes is highly significant: p < 0.001. D, Columns show the percentage of cultures showing preferential outgrowth on caudal membranes treated with PI-PLC enzyme or buffer. Numbers above each column indicate number of cocultures observed for each category. A χ2 test for differences between rostral and caudal neurite outgrowth on PI-PLC enzyme or buffer treated caudal membranes is not significant: p = 0.10.