Figure 8.

Positional-dependent cervical spinal neurites outgrowth on membranes isolated from rostral or caudal halves of the diaphragm muscle. The cervical region of the E15 rat spinal cord was isolated and cut into individual segments from C₁ to C₈. Cervical cord slices were cocultured on alternating lanes of membranes from rostral diaphragm (RD) or caudal diaphragm (CD) muscles as in Figure 7. A, The percentage of cultures in which neurites of each segment grew preferentially on rostral diaphragm membranes (rostral sectors, filled squares) or caudal diaphragm membranes (caudal sectors, open circles). B, The same data as in A, but includes those cocultures where growth was “random” (open triangles).