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. 2007 Dec 19;27(51):13997–14006. doi: 10.1523/JNEUROSCI.3885-07.2007

Figure 2.

Figure 2.

A, Representative RT-PCR analyses of total RNA isolated from the SN of antiGCL transgenics versus wild-type littermates to assess dox-inducible expression of transgenic mRNAs. 1, Constitutive expression of 551 bp rTta mRNA in dox- and no-dox-fed transgenics but not in wild-type littermates (Dox, antisenseGCL + dox; ND, antisenseGCL − dox; WT, nontransgenic littermate). 2, Inducible expression of 337 bp luciferase mRNA in dox-fed but not no-dox-fed antisenseGCL transgenics (Dox, antisenseGCL + dox; ND, antisenseGCL − dox; WT, nontransgenic littermate + dox). B, ICC analysis of luciferase marker expression in TH+ SN neurons (green, TH; red, luciferase; yellow, merged; Dox, antisenseGCL + dox; ND, antisenseGCL − dox; WT, nontransgenic littermate). Original magnification, 20×. C, Measurement of transgenic SN luciferase activity in various dox-fed antisenseGCL transgenic founder lines. WT, Nontransgenic littermate; ND, no-dox-fed antisenseGCL transgenic; HZ1-HZIV, dox-fed antisenseGCL transgenic founder lines. Note that the HZ-II line, which gave the highest transgene induction versus ND, was used for all subsequent studies. *p < 0.05 versus WT control.