Figure 7.

Effect of age-related glutathione alterations on ST DA content and on SN TH⁺ cell numbers in dox- versus no-dox-fed young and old mice. A, Striatal dopamine levels were measured in transgenic antisenseGCL versus wild-type mice via reverse-phase HPLC (n = 6 mice per group) in young (3 months) and old (12 months) mice. Values represented as picomoles of DA per milligram of protein. *p < 0.05, dox antisenseGCL versus wild-type or no-dox-fed mice. B, Cell counts of SN TH⁺ cell numbers after GSH depletion demonstrate a significant loss of DA SN neurons in old mice (12 months). Cells were counted from a total of 15–20 sections in each field per brain at a magnification of 100× using the optical fractionator approach. n = 5 animals per group. *p < 0.01, dox antisenseGCL versus WT or ND antisenseGCL.