Figure 2.

Activation of STATs by NPC cell CM assessed by reporter gene assays in HepG2 cells. Cells were transfected with a reporter construct containing each response element. A, B, At 24 h after transfection, they were stimulated for 6 h with recombinant cytokines (A) or CM of H34 or UCH cells (B). The concentration of each cytokine was 10 ng/ml. In C, UCH CM was incubated with neutralizing antibodies at 37°C for 1 h before application. The concentration of each antibody was 2 μg/ml. Each bar represents the mean ± SEM of three determinations, each performed in triplicate. *p < 0.01, significantly different from the values without neutralization.