Figure 5.

Reelin inhibition alters the location of SGZ proliferating cells in hippocampal slices. a, BrdU-labeled cells (arrows) are located mainly at the border of the granule cell layer (gcl) and hilus (h) in an organotypic hippocampal (HC) slice culture derived from a P14 mouse and cultured for 48 h. The mouse was given a single BrdU injection 2 h before slice preparation. The granule cell layer is made visible by a light bizbenzimide counterstain. b–g, BrdU labeling in different hippocampal slices from a single mouse cultured for 48 h with vehicle (Veh; b–d) or RAP (e–g) to block Reelin receptors. BrdU labeling is confined mainly to the hilar/granule cell layer border (dashed arrows) of controls (b–d), but BrdU-immunoreactive nuclei are scattered throughout the hilus and molecular layer (ml) in RAP-treated cultures (e–g). h, Schematic diagram of the experimental design. Scale bar, 50 μm.