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Schematic of experimental design showing times of brain slice preparation (arrows) and recording (bars) relative to LH surge induction in OVX+E mice. No surges occur in OVX mice; for OVX groups, times of slice preparation and recording were matched to times of negative feedback (red), surge onset (blue), and surge peak (green) states in OVX+E mice for comparison. The LH surge profile is adapted from mean values reported by Christian et al. (2005).
