Figure 6.
Kv4.3 siRNA broadens action potentials and inhibits MPOs in interneurons in slice cultures. A, Representative traces of spontaneous action potentials in interneurons illustrating that Kv4.3 siRNA (top) and 4-AP (bottom) increase action potential duration. B, Summary bar graphs indicating that APDhalf-width was significantly increased by either Kv4.3 siRNA compared with control siRNA or by 5 mm 4-AP (left), whereas action potential amplitude was unaffected by Kv4.3 siRNA or by 4-AP (right). C, Representative traces (in presence of CNQX, AP5, and bicuculline) and corresponding power spectra for the same cell illustrating MPOs and the inhibitory effect of the K+ channel blocker 4-AP (5 mm). D, Summary bar graphs indicating that the power (left) but not the peak frequency (right) of MPOs is reduced by hyperpolarization, TTX, and 4-AP. E, Representative traces and corresponding power spectra for the same cells showing that MPOs are decreased in interneurons transfected with Kv4.3 siRNA (bottom) compared with interneurons transfected with control siRNA (top). F, Summary bar graphs demonstrating the significant reduction in MPO power (left) and unchanged peak frequency (right) by Kv4.3 siRNA compared with the control siRNA. *p < 0.05.