Figure 11.

IFN-γ induction of chemokines. A, Differentiated oligodendrocyte cultures were stimulated with various doses of IFN-γ (0–100 U/ml) for 48 h, and the conditioned media were assayed for cytokine production by using LiquiChip assay. Control unstimulated oligodendrocytes produced no detectible levels of cytokines or chemokines. Oligodendrocyte stimulated with IFN-γ upregulated the expression of a number of chemokines: IP10, RANTES, MIP-1α, and MCP-1. No expression of IL1β, IL-6, and TNF-α was found in any of the samples. B, IP10 expression levels were measured in the spinal cords of CFA-injected controls and immunized wild-type (WT) and PLP/SOCS1 mice with identical clinical scores (0.0, 1.0, and 3.0) using Q-PCR. We found significantly lower expression of IP10 in the PLP/SOCS1 mice with a clinical score of 3.0 compared with the wild-type mice with an identical disease severity (*p < 0.05).