Figure 5.

Composition of the inflammatory foci in wild-type and PLP/SOCS1 mice. Cell composition of inflammatory foci was assessed in anti-CD3 and anti-F4/80 immunostained and H&E-stained PLP/SOCS1 and wild-type spinal cords. No qualitative difference in the composition of the inflammatory foci was found. A, B, Three quantitative differences were found: PLP/SOCS1 mice had a higher number of CD3⁺ and F4/80⁺ cells at days 10 and 15 PI compared with the corresponding time points in the wild-type mice (*p < 0.05) but fewer CD3⁺ and F4/80⁺ cells at disease onset compared with the wild-type mice at days 10 and 15 PI, respectively (**p < 0.05), and fewer F4/80⁺ cells at disease peak at days 12 and 18 PI, respectively (***p < 0.05). C–N, Representative images of immunostained (C–H) (scale bar, 25 μm) and H&E-stained (I–N) (scale bar, 50 μm) spinal cords of PLP/SOCS1 (first and third row; C–E, I–K) and wild-type (WT) (second and fourth row; F–H, L–N) mice with identical clinical scores of 0.0 (first column; C, F, I, L; CFA controls), 1.0 (second column; D, G, J, M), and 3.0 (third column; E, H, K, N). Note that the inflammatory foci comprise predominantly mononuclear, CD3/FITC-positive (green) and F4/80/Cy3-positive (red) cells.