Figure 7.

Patterns of oligodendrocyte apoptosis in wild-type and PLP/SOCS1 mice. Representative images of oligodendrocyte apoptosis in CNS: PLP/SOCS1 (top row; A–E) and wild-type (WT) spinal cords (bottom row; F–J) with identical clinical scores of 0.0 (first column; A, F, CFA control), 1.0 (second and third columns; B, G corresponding to C, H), and 3.0 (fourth and fifth column; D, I corresponding to E, J) were stained with TUNEL/FITC (green), CC1/Cy3 (red), and DAPI (blue). Stars mark the inflammation and the dotted line represents its edge; arrows point to the apoptotic oligodendrocytes. Scale bar, 100 μm. Note that apoptotic oligodendrocytes in the PLP/SOCS1 mice were localized at some distance from the edge of the inflammatory lesions, whereas in the wild-type mice they were within the lesion.