Figure 3.

Pathogenic parkin mutations decrease the NF-κB-activating potential of parkin. HEK293T cells cotransfected with the NF-κB reporter plasmid and either wt parkin or the parkin mutants indicated were harvested 24 h after transfection. Shown is the fold induction of luciferase activity compared with the EYFP control. *p < 0.01, **p < 0.001, ***p < 0.0001 compared with cells expressing wt parkin. As a control for parkin expression, an aliquot of the cell lysates was immunoblotted with the anti-parkin pAb hP1 (bottom panels). α-Tubulin was used as a loading control.