Figure 9.

IKKγ and TRAF2 colocalize with parkin. SH-SY5Y cells attached to coverslips were transfected with wt parkin and FLAG-tagged IKKγ or TRAF2. One day after transfection, cells were fixed, permeabilized, and stained with the following antibodies: anti-parkin hP1 pAb (red) and anti-FLAG mAb (green). DAPI was added to the mounting medium to label nuclei. Red arrows indicate the regions in which intensity profiles (bottom panels) along a line were determined using Leica confocal software version 2.6.1. Scale bars, 10 μm