Figure 1.
E2 reduces Aβ-induced neuronal death in a dose- and time-dependent manner. A, Neuron cultures were pretreated with increasing concentrations (0.001–1000 nm) of E2 for 60 min, followed by exposure to 25 μm Aβ25–35 for 48 h, and then assayed for cell viability. Data show mean cell viability (+SEM) from a representative experiment (n = 6). Significance is defined as follows: *p < 0.01 compared with Aβ25–35 condition. B, Neuron cultures were pretreated with 0 nm (black bars) or 10 nm (gray bars) E2 for 60 min, followed by exposure to 25 μm Aβ25–35 for the indicated times, and then assayed for cell viability. Data show mean cell viability (+SEM) from a representative experiment (n = 6). Significance is defined as follows: *p < 0.01 compared with Aβ treatment at the matched time point.