Figure 3.
Estrogen attenuates Aβ-induced Bcl-w downregulation and Bim upregulation. A, B, Neuron cultures were pretreated with 10 nm E2 for 60 min, followed by exposure to 25 μm Aβ25–35 for indicated times, and analysis of bcl-w (A) and bim (B) mRNA expression by RT-PCR. β-actin served as an internal control. C, Neuron cultures were pretreated with 10 nm E2 for 60 min followed by exposure to 25 μm Aβ25–35 for 48 h. A representative Western blot of culture lysates shows protein levels of Bcl-w (top panel) and Bim (middle panel); β-tubulin (bottom panel) was used as a control. D, E, Relative amounts of Bcl-w (D) and Bim (E) protein levels were determined by densitometric scanning of Western blots from three to four independent experiments. Data are represented as a mean (+SEM) percentage of control values. #p < 0.01 compared with vehicle-treated control group; *p < 0.01 relative to Aβ25–35-treated condition.