Figure 7.

Effect of insulin on tau phosphorylation during streptozotocin-induced diabetes. Proteins from mouse brain hemispheres (hippocampus plus cortex) were separated by SDS-PAGE and identified with the antibodies listed on the left of each blot. Ten days of STZ treatment (lanes 3 and 4) induced a slight hyperphosphorylation of tau at all the epitopes studied when compared with controls (lanes 1 and 2). Implantation of subcutaneous pumps delivering 0.75 U/d of insulin rescued tau phosphorylation to control levels (lanes 5 and 6). Each lane represents an individual mouse. INS, Insulin.