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. 2007 Dec 12;27(50):13706–13718. doi: 10.1523/JNEUROSCI.3503-07.2007

Figure 4.

Figure 4.

Synaptic insertion of GluR1 is not sufficient to drive spine enlargement. A, B, Expression of PSD-95 increases SEP-GluR1 enrichment on spines. A, Sample images of spines expressing SEP-GluR1 with or without PSD-95. Images are pixelwise ratio of green (SEP-GluR1)/red (volume). The color bar is shown at the right of images with red depicting high G/R and blue depicting low G/R. B, Cumulative distribution of spine SEP-GluR1 enrichment with or without PSD-95. Enrichment = [(spine integrated green/spine integrated red)/(dendrite mean green/dendrite mean red)]. +PSD-95, 15.9 ± 2.8, n = 3 cells, 196 spines; −PSD-95, 2.8 ± 0.1, n = 4 cells, 302 spines; p ≪ 0.01. Log scale was used to capture the distribution details across the full range of both data sets. C, Cumulative distribution of spine volume from cells expressing SEP-GluR1 with or without PSD-95. Spine volume is defined as (integrated spine red fluorescence)/(mean dendritic red fluorescence). +PSD-95, Median volume, 38.8, n = 3 cells, 196 spines; −PSD-95, median volume, 36.4, n = 3 cells, 200 spines; p = 0.27. D1, D2, Neurons expressing SEP-GluR1, untagged-PSD-95, and tDimer (n = 196 spines; 3 cells). D1, Mean spine volume (integrated red fluorescence) and spine SEP-GluR1 (integrated green fluorescence) relative to chemLTP induction. Values normalized to −10 min time point. *p < 0.05. E, Sample images obtained at indicated times relative to chemLTP induction (red channel only). Images are displayed as in Figure 2. Error bars represent SEM. Scale bars, 1 μm.