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. 2007 Dec 12;27(50):13706–13718. doi: 10.1523/JNEUROSCI.3503-07.2007

Figure 5.

Figure 5.

GluR1 C terminus (C-tail) peptide permits spine enlargement. A1, A2, Neurons expressing eGFP-GluR1-C-tail peptide and tDimer (n = 318 spines; 6 cells). A1, Mean spine volume (integrated red fluorescence) and spine GluR1-C-tail (integrated green fluorescence) relative to chemLTP induction. Values are normalized to −10 min time point (*p < 0.05). A2, Sample images obtained at indicated times relative to chemLTP induction (red channel only). Images are displayed as in Figure 2. B1, B2, Same as A for neurons expressing eGFP-GluR1(T887A)-C-tail peptide (n = 270 spines; 6 cells). B3, Paired whole-cell recordings of AMPAR- and NMDAR-mediated currents from uninfected cells and neighboring infected cells expressing GFP-GluR1 C-tail (T887A) (n = 10). Recordings are performed as in Figure 2. B4, LTP is blocked in cells expressing GluR1(T887A)-C-tail peptide, compared with eGFP control, eGFP (n = 16), GluR1(T887A)-C-tail (n = 17). LTP induction as in Figure 2 (*p < 0.02). C, Cumulative distribution of fold spine volume change during chemLTP from cells expressing GFP-GluR1 C-tail (wt) or (T887A). Fold volume change is defined as in Figure 2. Error bars represent SEM. Scale bar, 1 μm.