Figure 5.

Cortical layers form normally in Itgb1-NEXko mice. a–f, Sagittal sections through the cerebral cortex of wild-type, Itgb1-CNSko, and Itgb1-NEXko mice at P60 were stained with antibodies to CUX1 (brown), to reveal layer II–IV neurons. CUX1-positive neurons in Itgb1-NEXko mice were confined to cortical layers II–IV. CUX1-positive neurons in Itgb1-CNSko mice were also present in cortical layers II–IV, but the layer had a wavy appearance and appeared to be broader than in wild-type mice. g–m, Sagittal sections through the cerebral cortex were stained with an antibody to a nonphosphorylated form of neurofilament (Smi32) (brown), which specifically stains a subset of layer III and V neurons, as well as a subset of cells in the hippocampus. Neurons in wild-type, Itgb1-CNSko mice, and Itgb1-NEXko mice were appropriately located in layer III and V, but layer in Itgb1-CNSko mice appeared wavy (g–i). Higher-magnification views revealed that dendritic process in layer III (j, k) and the hippocampus (l, m) were not noticeably affected in Itgb1-NEXko mice. Scale bars: a–c, 400 μm; d–f, 125 μm; g–i, 450 μm; j–m, 70 μm.