Figure 5.

The Mix-induced inhibition of astrocytic GJC is prevented by the inhibition of p38 MAPK but not by lowering intracellular redox state. a, Representative pictures depicting the diffusion of LY though GJC in confluent astrocyte cultures either under untreated condition (Control) or after 24 h treatment with Mix alone, Mix and 10 μm SB202190 (SB), or Mix and 10 mm DTT (DTT). Scale bar, 100 μm. b, c, Graph providing the quantification of the fluorescent area obtained with the SL/DT assay performed in astrocyte cultures treated for 24 h with Mix (b) or CM* (c). In each case, astrocytes were cotreated (or not) with 10 μm SB202190 (SB) or 10 mm DTT and compared with untreated conditions as control. Each value, expressed in AU, corresponds to the mean ± SEM obtained from seven independent measurements. n.s., No significant difference; ***p < 0.001.