Figure 8.

Proinflammatory treatment increases glucose uptake in astrocytes. a, Confluent astrocyte cultures were treated with CM* for 24 h, and then uptake of 2-NBDG, a fluorescent glucose derivative, was determined at 488 nm. a, Snapshot pictures of fields showing nuclei staining of astrocytes treated as indicated for each panel. Scale bar, 100 μm. b, Graph showing the uptake of 2-NBDG expressed as arbitrary fluorescent units in astrocytes under control conditions (Control) or treated for 24 h with MG conditioned medium (CM*). In some experiments, cells were treated with either La³⁺ (200 μm) or gap27 (300 μg/ml) for 10 min before dye uptake assay. Each plotted value represents the mean ± SEM of four independent determinations. n.s., No significant difference; *p < 0.05 and ***p < 0.001.