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. 2007 Dec 12;27(50):13719–13729. doi: 10.1523/JNEUROSCI.3006-07.2007

Figure 2.

Figure 2.

Effect of concentration and time of exposure on Aβ binding to cells. PC12 cells were exposed for different periods of time to different concentrations of Aβ-FITC, and Aβ-FITC binding was analyzed by EPICs XL-MCL. A, Histograms of the number of cells versus Aβ-FITC fluorescence after 60 min exposure to different concentrations of Aβ-FITC. B, Percentages of cells (filled squares) and fluorescence intensity [geometric mean of fluorescence (GeoMean of Fluor), filled circles] of bound Aβ-FITC (M2 gate from A) as a function of the concentration of Aβ-FITC. C, Histograms of the number of cells versus Aβ-FITC fluorescence at different times of exposure to 2 μm Aβ-FITC. D, Percentages of cells with bound Aβ-FITC (M2 gate from C) as a function of time of exposure to 2 μm Aβ-FITC. Binding and the percentage of cells that bind Aβ-FITC increase as a function of time of exposure and concentration of Aβ to reach a saturation level.