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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Bone. 2016 Sep 4;92:180–188. doi: 10.1016/j.bone.2016.09.001

Figure 3. The ECR5 enhancer is mechanosensitive.

Figure 3.

(A) Diagram of Luciferase plasmids under control of heterologous SV40 promoter, the 2kb human SOST promoter, in the absence of presence of three copies of ECR5. (B) Oscillatory fluid flow significantly increased Luciferase activity in cells transfected with heterologous SV40 or SOST promoter, whereas plasmids containing ECR5 decreased Luciferase activity in response to fluid flow. (C) Time course of suppression of ECR5-driven Luciferase activity. (D) Dose-dependent effect of ECR5 copy number on Luciferase activity. Different letters denote statistical differences among groups.