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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Neurobiol Dis. 2018 Jun 22;118:22–39. doi: 10.1016/j.nbd.2018.06.015

Fig. 10. GSK-3β inhibition by AR-A014418 suppresses and Wnt3A treatment enhances Notch signaling.

Fig. 10.

A) Representative Western blot analyses for NICD from pups without IVH, AR-A014418-treated and vehicle treated pups with IVH. Adult rat brain was employed as positive control. Each lane represents lysate from a whole coronal slice taken at the level of mid septal nucleus of one brain. Bar chart shows mean ± s.e.m. (n=5 each group). NICD levels were reduced in AR-A014418 treated pups relative to vehicle controls. mRNA expression of Hes5 levels were reduced in AR-A014418 treated pups compared to vehicle controls at D7, but not at D3. Hes1 mRNA expression was similar between groups. B) Representative Western blot analyses for NICD from pups without IVH, Wnt3A-treated and vehicle-treated pups with IVH. Adult rat brain was employed as positive control. Each lane represents lysate from a whole coronal slice taken at the level of mid septal nucleus of one brain. Bar chart shows mean ± s.e.m. (n=5 each group). NICD levels were elevated in Wnt3A treated pups relative to vehicle controls. mRNA expression of Hes1 levels were elevated in Wnt3A treated pups compared to vehicle controls at D3, but not at D7. Hes5 mRNA expression was similar between groups. *P<0.05, **P<0.01 pups with vs. without IVH. #P<0.05, ## P<0.01 vehicle vs. rh-Wnt3A treated pups with IVH.