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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Neurobiol Dis. 2018 Jun 22;118:22–39. doi: 10.1016/j.nbd.2018.06.015

Fig. 5: GSK-3β inhibition by AR-A014418 improves myelination in rabbits with IVH:

Fig. 5:

A) Representative immunofluorescence of myelin basic protein (MBP) in the corona radiata of D 14 pups. Data are mean ± s.e.m. (n = 8 each group). Volume fractions of MBP were elevated in the corpus callosum and corona radiata of AR-A014418 treated pups compared with vehicle controls with IVH. Scale bar, 200 μm. V, ventricular side. B) Typical Western blot analysis for MBP in the forebrain of premature rabbit pups, as indicated, at D14. Adult rat brain was employed as positive control. Each lane represents lysate from a whole coronal slice taken at the level of mid septal nucleus of one brain. Bar chart shows mean ± s.e.m. (n=5 each group). MBP expression was higher in AR-A014418 treated pups compared with vehicle treated pups. C) Western blot analysis for MAG in the forebrain of pups as indicated at D14. Adult rat brain was used as positive control. Bar graph shows mean ± s.e.m. (n=5 each group). MAG expression was elevated in AR-A014418 treated pups compared with vehicle controls. D) Representative electron micrograph from rabbit pups without and with IVH, and pups with IVH treated with AR-A014418 at d 14. Note that myelinated axons were significantly decreased in pups with IVH compared to controls without IVH and that AR-A014418 treatment shows a trend towards increase in myelinated axons in pups with IVH. ***P<0.001 pups with vs. without IVH. #P<0.05, ###P<0.001 vehicle vs. AR-A014418 treated pups with IVH. Scale bar, 1 μm. no IVH, glycerol treated no IVH; IVH, IVH after glycerol treatment. No IVH implies glycerol no IVH.