Table 2.
List of primers used in qPCR for 10 putative genes related to spore germination and a reference gene (18S ribosomal RNA).
|
A. oryzaer (Gene ID number) |
Putative gene function | Primer sequences |
|---|---|---|
| AO090005001280* | Extracellular thaumatin domain protein, putative | Forward-TCCCTCTGTCAACTGTGCTG |
| Reverse-TGCTGACACTGAAGGAGGTG | ||
| AO090011000630 | Carbamoyl-phosphate synthase, large subunit | Forward-TCGAATGGTAAGCTCCTGCT |
| Reverse-TCAGCAATCTCCTTGACACG | ||
| AO090011000649 | Purine-cytosine permease | Forward-CGTTTTGGCCTGTATTGGTT |
| Reverse-TAGCACACCTGCAAATCCTG | ||
| AO090005000164 | Conserved hypothetical protein | Forward-CCATCTCAACGTCTCAAGCA |
| Reverse-CCAACGACAAGAAGTGAGCA | ||
| AO090011000497 | Hypothetical nuclear protein | Forward-TACACCGATGGAGGGAGTTC |
| Reverse-AGAGGGTTACTGCTGCCTGA | ||
| AO090001000546 | 5′-methylthioadenosine phosphorylase | Forward-AGGCTACCGCAGATGTCACT |
| Reverse-CGAACTTGACAGAGCCTTCC | ||
| AO090026000687 | MYB DNA binding protein (Tbf1), putative | Forward-ACATCATCAGGAAGGCCAAC |
| Reverse-GGCAATGAAAGCCTGTGTTT | ||
| AO090003000611 | Hydroxymethylglutaryl-CoA synthase | Forward-TGCTCGTCCTCAGAACATTG |
| Reverse-TGTCCCAGACCGATTGTGTA | ||
| AO090003001496 | Conserved hypothetical protein | Forward-CCACTGGCCTCACTACCATT |
| Reverse-TACAGGTTGAGGGCCAAGAC | ||
| AO090003000685 | ATF/CREB family transcription factor, AtfA | Forward-GTCGCCAGAGGAAGAAACAG |
| Reverse- AGAAACCGGGCAGTCCTTAT | ||
| AO090206r00001 | 18S ribosomal RNA | Forward-GGAAACTCACCAGGTCCAGA |
| Reverse-AGCCGATAGTCCCCCTAAGA |
*Ten putative genes related to A. oryzae spore germination were selected for qPCR based on the report by Hagiwara et al.41; genes with more than a 20-fold fragments per kilobase of transcript per million mapped reads (FPKM) ratio in 1 h-grown conidia compared with conidia, as detected by RNA-sequencing analysis.