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. 2019 Aug;29(8):1352–1362. doi: 10.1101/gr.243477.118

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© 2019 Meleshko et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — The biosyntheticSPAdes pipeline. Six steps of the biosyntheticSPAdes pipeline: (1) assembling genomic/metagenomic reads with SPAdes/metaSPAdes; (2) searching for edges harboring biosynthetic domains in the assembly graph; (3) extracting biosynthetic gene cluster subgraphs from the assembly graph; (4) restoring the collapsed domains in the BGC-subgraphs; (5) constructing the scaffolding graph; and (6) generating putative BGC by solving the Rural Postman Problem in the scaffolding graph.