Figure 2.

The spontaneous electrical activity of GHRH-GFP neurons. A, B, Raw extracellular recordings performed in GHRH neurons with the loose-patch clamp technique. Calibration: A, 200 pA, 2 s; B, 50 pA, 5 s. C, D, Instantaneous frequency of action potentials recorded with the loose patch clamp in a male and female GHRH-GFP neuron, respectively. E, F, Cumulated histograms of the mean instantaneous frequency of action potentials recorded in male (E, diamonds; n = 104) and female (E, squares; n = 121) GHRH-GFP neurons, as well as in female GHRH-GFP neurons (F) during diestrus (DiE), proestrus (ProE), oestrus (E), and metestrus (MetE). ns, Not significant.